DESCRIPTION

• Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable to measure fluorescence polarization Adjustable micropipettor and sterile tips 1,4-Dithiothreitol (DTT) 1 M in anhydrous DMSO
• Species: Dog
• Shiptemp: -80°C (dry ice)
• Synonyms: inhibitor screening, assay kit, PDE3A
• Warnings: Avoid freeze/thaw cycles.
• Category: Phosphodiesterase/Assay Kit
• Background: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE3A, also known as cGMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.
• Description: The Dog PDE3A Assay Kit is designed for identification of inhibitors of canine PDE3A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Dog PDE3A to the binding agent.
• Methodology: Fluorescence Polarization
• Supplied As: The Dog PDE3A Assay Kit comes in a convenient 96-well format, with purified Dog PDE3A1 enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions.
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 60706, 60036, 79606, 79736
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Phosphodiesterase
• Instructions for Use: See assay kit datasheet for detailed protocol.