Anti-ATG5 (Autophagy Marker) Monoclonal Antibody(Clone: ATG5/2101)

DESCRIPTION

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjµgating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.

DETAILS

Gene: ATG5
App Id: ELISA,WB,IHC
Gene Id: 9474
Isotype: Mouse IgG1, kappa
Clone Name: ATG5/2101
Uniprot Id: Q9H1Y0
Host Animal: Mouse
Clonality Id: Monoclonal
Reactivity Id: Human
Product Content: 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Alternative Name: Autophagy protein 5 (APG5); APG5 autophagy 5 like (APG5L); Apoptosis-specific protein (ASP); ATG5; Autophagy related 5; hAPG5
Application Note: ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA); Western Blot (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Immunogen Information: Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)