DESCRIPTION

• Mw: 1611 Da
• Form: Lyophilized
• Cas No: 271246-66-3
• Purity: ≥98% (HPLC)
• Source: Synthetic peptide
• Target: FPR2 receptor
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: FPR2
• Activity: MMK1 is a selective and potent agonist of the formyl peptide receptor (FPR2)1.
• Is Toxin: No
• Sequence: LESIFRSLLFRVM-OH
• Synonyms: FPR2 agonist
• Lead Time: 1-2 Business Days
• Solubility: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
• Formulation: Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
• Bioassay Tested: yes
• Molecular Formula: C75H123N19O18S1
• Storage of Solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
• Steril Endotoxin Free: no
• Effective Concentration: 1 nM - 10 μM
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
• Reconstitution and Solubility: Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
• Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
• Product Page - Scientific Background: Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.MMK1 is a selective and potent agonist of the Formyl peptide receptor FPR23, which was originally derived from a random peptide library and was identified by a novel autocrine selection method in yeast engineered to express human FPR24.FPR2 is expressed in the promyelocytic leukemia cell line HL-60 as well as in the chronic myelogenous leukemia cell line K5625. In human neutrophils, 1 µM MMK1 induces Ca2+ influx which is blocked by the specific FPR2 antagonist WRW46.Resveratrol, a constituent of grape seeds, induces Ca2+ influx in human monocytes which is blocked by 10 µM MMK1, demonstrating that the inhibition of chemoattractant receptors contribute to the anti-inflammatory properties of resveratrol7.