DESCRIPTION

• Aa: 2-507(end)
• Mw: 84 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_001197221
• Species: Human
• Uniprot: Q08499
• Shiptemp: -80°C (dry ice)
• Synonyms: PDE4D2, phosphodiesterase 4D2
• Warnings: Avoid freeze/thaw cycles.
• Category: Phosphodiesterase/Enzymes
• References: 1. Fidani, L., et al., Eur. J. Neurol. 14 (7):745-749 (2007).2. Baillie, G.S., et al., Biochem. J. 404 (1):71-80 (2007). Application Reference(s): 1. Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015) 2. Evaluation of the therapeutic index of a novel phosphodiesterase 4B-selective inhibitor over phosphodiesterase 4D in mice (2013) 3. Synthesis of 2,2,4-trimethyl-1,2-dihydroquinolinyl substituted 1,2,3-triazole derivatives: their evaluation as potential PDE 4B inhibitors possessing cytotoxic properties against cancer cells (2014)
• Description: Human PDE4D2 (GenBank Accession No. NM_001197221), a.a. 2-507(end) with N-terminal GST tag, MW=84 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
• Assay Conditions: 10 mM Tris-HCl,_x000D_ pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,_x000D_ 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and_x000D_ serial dilutions of PDE4D2 at 37°C for 20 min._x000D_ Quantified by 5'- nucleotidase cleaving the_x000D_ 5' -AMP product and releasing the_x000D_ phosphate group which is detected by_x000D_ Malachite Green Reagent.
• Specific Activity: ≥ 13,600 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Phosphodiesterase