SMARCA4 Antibody

DETAILS

Buffer: SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Isotype: IgG
Storage: SMARCA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Category: Antibody
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Shipping: Blue Ice
Clonality: Polyclonal
Conjugate: Unconjugated
Immunogen: SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4.The immunogen is located within amino acids 1420 - 1470 of SMARCA4.
Swissprot: P51532
User Note: Optimal dilutions for each application to be determined by the researcher.
Background: The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1). Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2). The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3). In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4,5).
Specificity: SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.
Host Species: Rabbit
Ncbi Gene Id: 6597
Purification: SMARCA4 antibody is affinity chromatography purified via peptide column.
Concentration: 1 mg/mL
Modifications: None
Ncbi Organism: homo sapiens
Physical State: Liquid
Additional Names: SMARCA4 Antibody: BRG1, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB, hSNF2b, BAF190A, BRG1, SNF2B, Transcription activator BRG1, ATP-dependent helicase SMARCA4
Application Note: SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
By Research Area: Homeostasis
Protein Accession: NP_001122321
Protein Gi Number: 192807323
Ncbi Official Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate
Species Reactivity: Human,Mouse,Rat
Tested Applications: ELISA,WB,IHC-P,IF
Ncbi Official Symbol: SMARCA4
Predicted Molecular Weight: Predicted: 185 kDa Observed: 200 kDa