SPN-C82E9-25ug SPN-C82E9-200ug
Biotinylated SARS-CoV-2 S protein, His,Avitag™, Super stable trimer (MALS verified)
ACROBiosystems
Image
![](https://www.acrobiosystems.com/static/main/products/images/sds/SPN-C82E9.jpg)
Image
![](https://www.acrobiosystems.com/static/main/products/images/elisa/SPN-C82E9-E1.jpg)
Image
![](https://www.acrobiosystems.com/static/main/products/images/elisa/SPN-C82E9-E2.jpg)
Image
![](https://www.acrobiosystems.com/static/main/products/images/spr/SPN-C82E9-S1.jpg)
DESCRIPTION
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
DETAILS
- Tag: C-10×His & C-Avi
- Host: HEK293
- Size: 25ug
- Buffer: PBS
- Format: Powder
- Purity: 95%
- Source: Biotinylated SARS-CoV-2 S protein, His, Avitag, Super stable trimer (SPN-C82E9) is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
- Storage: -20℃
- Category: MABSol® Biotin Labeled Proteins
- Molecule: Spike protein
- Accession: QHD43416.1
- Conjugate: Biotin-labeled
- Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Exp Region: Val 16 - Pro 1213
- Package Size: 25ug*1
- Characteristics: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 139.7 kDa. The protein migrates as 180-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Endotoxin Level: 1.0 EU per μg
- Molecular Weight: 139.7 kDa
- Shipping Condition: RT
- Species Reactivity: SARS-CoV-2
- Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html