DESCRIPTION

• Aa: 2-363(end)
• Mw: 40 kDa
• Tags: N-terminal His-Tag
• Format: Aqueous buffer solution.
• Purity: ≥90%
• Genbank: NM_017825.3
• Species: Human
• Uniprot: Q9NX46
• Shiptemp: -80°C (dry ice)
• Synonyms: ADP-ribosylhydrolase ARH3, ADP-ribose glycohydrolase ARH3, ADP-ribosylhydrolase 3, O-acetyl-ADP-ribose deacetylase ARH3, Poly(ADP-ribose) glycohydrolase ARH3, ADPRHL2, ARH3
• Warnings: Avoid freeze/thaw cycles
• Category: DNA Replication/Protein
• Background: ADPRS, also known as ADP-ribosyl-acceptor hydrolase 3 or ARH3, is part of the DNA damage response machinery, and removes ADP-ribose from serine residues in a Mg2+-dependent manner. It acts sequentially to PARG (poly(ADP-ribose) glycohydrolase) and it has a protective role by decreasing the levels of PAR in the cell, which stops mitochondria from releasing PAR-driven AIF (apoptosis inducing factor). Mutations that result in loss of function of this protein lead to CONDSIAS (stress-induced childhood-onset neurodegeneration with variable ataxia and seizures), a disease with multiple clinical expressions. The development and use of ADPRS inhibitors allows us a better understanding of the DNA damage response pathway and opens new avenues for cancer treatment.
• Description: Recombinant human ADPRS (ADP-ribosyl-acceptor hydrolase 3, or ARH3), full length encompassing amino acids 2-363(end). This construct contains an N-terminal His-tag (6xHis). This protein was affinity purified.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Host Species: E. coli
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Various concentrations of ARH3 were incubated at room temperature with 4 µM TFMU-ADPr (substrate) and the fluorogenic product was measured after 1 hour (λexcitation: 385 nm / λemission: 502 nm). 
• Related Products: 80584, 78858, 101720
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: DNA Replication and Repair
• Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.