DESCRIPTION

• Aa: full length
• Mw: 22.7 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_005809
• Species: Human
• Uniprot: P32119
• Shiptemp: -80°C (dry ice)
• Synonyms: PRDX2
• Warnings: Avoid freeze/thaw cycles.
• Category: Metabolic Enzymes/Protein
• References: 1. Peskin,A.V., et al., J. Biol. Chem. 282 (16), 11885-11892 (2007)2. Low,F.M., et al., Blood 109 (6), 2611-2617 (2007)
• Description: Human Peroxiredoxin 2, also known as PRDX2, full length (Genbank accession number NM_005809) with N-terminal His-tag, MW=22.7 kDa, expressed in Baculovirus infected Sf9 cell expression system.
• Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1.35 mM KCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of biochemical function, enzyme kinetics, screening inhibitors, and selectivity profiling.
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 3.45 µg of PRDX2 was incubated in 50 mM HEPES (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30°C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
• Specific Activity: ≥149 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Peroxiredoxin