3CL Protease (P252L) (SARS-CoV-2) Assay Kit

DESCRIPTION

The 3CL Protease (P252L) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic based assay designed to measure the activity of P252L mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. 3CL inhibitor GC376 is also included as a control. 3CL inhibitor GC376 is also included as a control. The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience #79952).

DETAILS

Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com
Mutation: P252L
Shiptemp: -80°C (dry ice)
Synonyms: main protease kit, Mpro, SARS-CoV-2 3CL Protease kit, untagged 3CL kit, 3 CL assay, 3CL Pro kit, nsp5
Warnings: Avoid freeze/thaw cycles
Category: Coronavirus/Assay Kit
Background: Coronaviruses (CoVs) cause respiratory and intestinal infections in humans and animals. The 3CL protease, also known as Main Protease (Mpro), plays a vital role in processing the polyproteins that are translated from the viral RNA. Protease inhibitors that can block viral replication are promising potential drug candidates for the treatment of patients suffering from COVID-19 infection.
Description: The 3CL Protease (P252L) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic based assay designed to measure the activity of P252L mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. 3CL inhibitor GC376 is also included as a control. 3CL inhibitor GC376 is also included as a control. The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience #79952).
Methodology: Fluorogenic
Supplied As: The kit contains enough purified 3CL Protease (P252L), fluorogenic substrate, and 3CL Protease assay buffer for 100 enzyme reactions.
Unspsc Code: 41105331
Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and High Throughput Screen (HTS) applications.
Product Type: Assay Kit
Biosafety Level: Not applicable (BSL-1)
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Scientific Category: Coronavirus