DESCRIPTION

• Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
• Host: Rabbit
• Clone: NA
• Label: Unconjugated
• Purity: Affinity purified on immobilized antigen.
• Target: Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: 11254
• Isotype: Rabbit IgG
• Homology: Mouse - 12/14 amino acid residues identical; rat - 11/14 amino acid residues identical
• Is Toxin: No
• Sequence: (C)PSEQYWNKVALQRS, corresponding to amino acid residues 213-226 of human SLC6A14
• Synonyms: Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14
• Accession: Q9UN76
• Clonality: Polyclonal
• Lead Time: 1-2 Business Days
• Reactivity: Human|Rat|Mouse
• Formulation: PBS pH7.4
• Applications: IF|IHC|WB
• Preservative: No Preservative
• Reconstitution: 0.2 ml double distilled water (DDW).
• Blocking Peptide: BLP-GT015
• Negative Control: BLP-GT015
• Positive Control: NA
• Immunogen Location: 2nd extracellular loop
• Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
• Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
• Immunogen Source Species: Human
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
• Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
• Applications May Also Work In: IF|IHC|WB
• Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
• Product Page - Scientific Background: SLC614A is an amino acid transporter which is a member of the Solute carrier (SLC) 6 transporter family. Currently, the SLC6 family is comprised of >300 prokaryotic and eukaryotic proteins, of which 21 have been identified in humans. SLC6 members have 12 membrane spanning domains (TM) with intracellular N- and C- termini1.SLC614A transports all amino acids (except glutamate and aspartate) into the cell. It has a uni-directional function and it is energized by three different driving forces: a Na+ gradient, a Cl- gradient and the membrane potential. These specific characteristics makes it important in the development of cancerous tumors2. During carcinogenesis, the developing tumor utilizes more resources than healthy tissue and, therefore, there is an over-production of transporters for glucose and amino acids. SLC614A is uniquely suited to the biological needs of tumor cells: Theoretically, this transporter has the ability to concentrate its substrates inside the cell ~1000-fold compared to the extracellular medium, which complies with the increasing demand of the tumor cell for amino acid supply2. Indeed, SLC614A has been shown to be up regulated in multiple cancer types such as, cervical cancer3, breast cancer4, and pancreatic cancer5, as well as the development of Cystic fibrosis6. Hence, the therapeutic potential in affecting the activity of this transporter is clinically significant.
• Standard Quality Control of Each Lot: Western blot analysis
• Application Dilutions Western Blot Wb: 1:200
• Antibody Concentration After Reconstitution: 1 mg/ml
• Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:200