BP1812a
ATG5 Antibody (N-term) Blocking peptide
Abcepta
DETAILS
- Format: Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
- Gene Id: 9474
- Category: Peptides; Blocking Peptides
- Subtitle: Synthetic peptide
- Gene Name: ATG5
- Other Names: Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5, APG5L, ASP
- Availability: 2 weeks
- Bio Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
- Bio References: Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)Hammond E.M., et al. FEBS Lett. 425:391-395(1998) Strausberg R.L., et al. PNAS 99:16899-16903(2002)Grand R.J.A., et al. Exp. Cell Res. 218:439-451(1995)Mizushima N., et al. J. Biol. Chem. 273:33889-33892(1998)Mizushima N., et al. J. Cell Biol. 152:657-668(2001)
- Primary Accession: Q9H1Y0
- Targetspecificity: The synthetic peptide sequence used to generate the antibody AP1812a was selected from the N-term region of human Autophagy APG5L. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.