Cost-effective dye-based qPCR Mix without passive reference dye ROX. High sensitivity and specificity Excellent efficiency Reaction set-up and shipment without dry ice Cost-effective solution for a wide range of applications Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. In this mix EvaGreen® double-stranded DNA binding dye is used instead of the more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen® dye shows a higher fluorescence level, high sensitivity for detecting low template concentrations, and high stability at room temperature. HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimized ready-to-use solution for dye-based real-time quantitative PCR assays on cyclers that do not require passive reference dye.

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