DESCRIPTION

• Form: Antigen Affinity Purified from Pooled Serum
• Gene: GRIN1
• Host: Rabbit
• Rrid: AB_2492169
• Type: Primary Antibody
• Buffer: Lyophilized
• Target: NMDA NR1 Subunit, Splice Variant N1
• Isotype: IgG
• Storage: Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
• Shipping: Blue Ice
• Synonyms: GluN1 antibody, Glutamate [NMDA] receptor subunit zeta-1 antibody, Glutamate receptor ionotropic N methyl D aspartate 1 antibody, Glutamate receptor ionotropic N-methyl-D aspartate subunit 1 antibody, glutamate receptor ionotropic NMDA 1 antibody, Grin1 antibody, MRD8 antibody, N methyl D aspartate receptor antibody, N methyl D aspartate receptor channel subunit zeta 1 antibody, N methyl D aspartate receptor subunit NR1 antibody, N-methyl-D-aspartate receptor subunit NR1 antibody, NMD-R1 antibody, NMDA 1 antibody, NMDA R1 antibody, NMDAR1 antibody, NMDA receptor 1 antibody, NMDA1 antibody, NMDAR antibody, NMDZ1_HUMAN antibody, NR1 antibody
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~120 kDa NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.
• Applications: WB, IHC
• Ncbi Gene Id: 24408
• Conjugate Exem: Unconjugated
• Physical State: Lyophilized
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 120
• Production Notes: Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
• Dilution Range Wb: 1:1000
• Immunogen Species: Rat
• Dilution Range Ihc: 1:1000 - 1:2000
• Species Reactivity: Mouse and Rat
• Target Description: The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
• Expected Reactivity: Canine, Chicken, Human, Non-Human Primate, Xenopus
• Dilution Range Wb Brain: 1:1000
• Uniprot Number Immunogen Species: P35439