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CD3-H5252-100ug CD3-H5252-1mg

Human CD73 Protein, Mouse IgG2a Fc Tag (active enzyme)

ACROBiosystems

DESCRIPTION

Human CD73, Mouse IgG2a Fc Tag (CD3-H5252) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).

DETAILS

  • Tag: C-mFc(mIgG2a)
  • Host: HEK293
  • Size: 100ug
  • Buffer: 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
  • Format: Powder
  • Purity: 95%
  • Storage: -20℃
  • Category: Enzymes
  • Molecule: CD73
  • Accession: NP_002517.1
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
  • Exp Region: Trp 27 - Ser 549
  • Swiss-prot: P21589-1
  • Characteristics: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 84.9 kDa. The protein migrates as 95-100 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
  • Endotoxin Level: 0.1 EU per μg
  • Molecular Weight: 84.9 kDa
  • Shipping Condition: RT
  • Species Reactivity: Human
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html