CT028-M08H
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Mouse IL-23 (IL23A & IL12B Heterodimer) Protein
Sino Biological
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DESCRIPTION
A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified.
DETAILS
- Tag: C-His
- Purity: > 95 % as determined by SDS-PAGE
- Source: HEK293 Cells
- Species: Mouse
- Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: 1. Immobilized Human IL23 Receptor hFc(Cat:13840-H02H) at 2 μg/ml (100 μl/well) can bind Mouse IL-23 His(Cat:CT028-M08H), the EC50 of Mouse IL-23 His is 50-250 ng/mL. 2. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-1 ng/mL. 3.Loaded Recombinant Human IL-23 Receptor Protein, hFc Tag (Cat. No. 13840-H02H) on proA Biosensor, can bind Recombinant Mouse IL-23 Protein, His Tag (Cat. No. CT028-M08H) with an affinity constant of 6.13 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4.Loaded Recombinant Rat IL-23 Receptor Protein, hFc Tag (Cat. No. 80395-R02H) on proA Biosensor, can bind Recombinant Mouse IL-23 Protein, His Tag (Cat. No. CT028-M08H) with an affinity constant of 93.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
- Category: Proteins
- Molecule: IL12B & IL23A
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4.
- Molecular Mass: The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: Q9EQ14 & P43432
- Predicted N Terminal: Val 22 & Met 23