40094
Scientist.com Supplier
CDK4(EE,T172A)/Cyclin D1, His-tag Recombinant
BPS Bioscience
DESCRIPTION
Complex of recombinant, full length human CDK4 (GenBank accession number NM_000075) with C-terminal His-tag and modifications (a.a. 43G-47G were replaced with EE, and T172A), and human Cyclin D1 (GenBank accession number NM_053056), a.a. 16-271, were co-expressed in a baculovirus-infected Sf9 cells expression system. CDK4 MW=34 kDa, Cyclin D1 MW=29 kDa.
DETAILS
- Aa: full length
- Mw: 63 kDa (complex)
- Tags: C-terminal His-tag (CDK4), no tag on Cyclin D1
- Format: Aqueous buffer solution
- Genbank: NM_000075(CDK4), NM_053056(cyclin D1)
- Species: Human
- Uniprot: P11802 (CDK4), P24385 (cyclin D1)
- Shiptemp: -80°C (dry ice)
- Synonyms: Cyclin-Dependent Kinase 4, Cell Devision Kinase 4, CDK4, Cyclin D1, CCD1, Bcl1, PRAD1, CCND1
- Warnings: Avoid freeze/thaw cycles.
- Category: Kinase-Serine/Threonine/Protein
- References: 1. Amendola D. et al., Cell Prolif. 2009. Feb; 42(1):94-109.2. Yang L. et al., Biochem Cell Biol. 2009. Dec; 87(6):933-42. Application Reference(s): 1. Iterative conversion of cyclin binding groove peptides into druglike CDK inhibitors with antitumor activity (2015)
- Description: Complex of recombinant, full length human CDK4 (GenBank accession number NM_000075) with C-terminal His-tag and modifications (a.a. 43G-47G were replaced with EE, and T172A), and human Cyclin D1 (GenBank accession number NM_053056), a.a. 16-271, were co-expressed in a baculovirus-infected Sf9 cells expression system. CDK4 MW=34 kDa, Cyclin D1 MW=29 kDa.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for three dimensional structure studies and binding assays.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 40104, 40102, 40454, 40206, 78487
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Kinase-Serine/Threonine