DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Warnings: Thalidomide is known to cause severe birth defects in humans. It is very important to use all appropriate precautions when handling this compound.
• Category: Ubiquitination/Assay Kit
• Description: The Cereblon Ubiquitination Homogeneous Assay Kit is designed to measure Cereblon auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled ubiquitin and proper A-screen beads to complete the pairing. This homogeneous assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
• Methodology: AlphaLISA®
• Supplied As: The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: Great for screening small molecular inhibitors or PROTACs for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 80412, 80415, 79770, 79790
• Contraindications: Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
• Storage Stability: At least six months from date of receipt when stored as directed.
• Scientific Category: PROTAC
• Instructions for Use: See assay protocol for detailed instructions.