mouse monoclonal IgG1; p-EGFR Antibody (9H2) is an IgG1 mouse monoclonal p-EGFR antibody that detects Tyr 1173 phosphorylated EGFR of mouse, rat and human origin by WB, IP and IF. p-EGFR Antibody (9H2) is available as the non-conjugated anti-p-EGFR antibody form. Epidermal growth factors mediate their effects on cell growth through interactions with a cell surface glycoprotein designated EGFR (EGF receptor). Binding of EGF or TGFα to EGFR activates tyrosine-specific protein kinase activity intrinsic to EGFR. The carboxy terminal tyrosine residues on EGFR, Tyr 1092 and Tyr 1173, designated Tyr 1196 in rat, are the major sites of autophosphorylation which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1092 and Tyr 1110 in human and mouse or Tyr 1109 in rat, and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1092 and Tyr 1110 is required for conformational change in the C-terminal tail of EGFR. Tyr 1092, Tyr 1173 and Tyr 1110 are also designated Tyr 1068, Tyr 1197, and Tyr 1086, respectively.

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.