52046
PRMT6 (HRMT1L6) Chemiluminescent Assay Kit
BPS Bioscience
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DESCRIPTION
The PRMT6 Direct Activity Assay kit is designed to measure PRMT6
activity for screening and profiling applications. The key to the PRMT6
Direct Activity Assay Kit is a highly specific antibody that recognizes methylated R3
residue of Histone H4. With this kit, only three simple steps on a microtiter plate are
required for methyltransferase detection. First, S-adenosylmethionine is incubated with a
sample containing assay buffer and methyltransferase enzyme for one hour. Next,
primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary
antibody followed by addition of the HRP substrate to produce chemiluminescence that
can then be measured using a chemiluminescence reader.
DETAILS
- Uniprot: Q96LA8
- Shiptemp: -80°C (dry ice)
- Synonyms: protein arginine N-methyltransferase 6, PRMT6, Histone-arginine N-methyltransferase, HRMT1L6
- Warnings: Avoid freeze/thaw cycles.
- Category: Methyltransferase/Assay Kit
- Background: PRMT6 is a histone methyltransferase that exhibits methylation activity toward H3-R2 and H4-R3.
- References: Dillon S.C., et al. 2005. Genome Biology 6:227.
- Description: The PRMT6 Direct Activity Assay kit is designed to measure PRMT6 activity for screening and profiling applications. The key to the PRMT6 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
- Methodology: Chemiluminescent
- Supplied As: Kit comes in a convenient format, with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine3 residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffe
- Unspsc Code: 41105332
- Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
- Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
- Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
- Scientific Category: Methyltransferase
- Instructions for Use: See assay kit datasheet for detailed protocol.