Skip to Main Content
Welcome to the Scientist.com Marketplace

Go to Main Navigation

SPN-C52Ht-50ug SPN-C52Ht-500ug

SARS-CoV-2 Spike Trimer Protein (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N), His Tag (MALS verified)

ACROBiosystems

DESCRIPTION

SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Ht) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). Mutations T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N were identified in the SARS-CoV-2 Delta sub-lineages (Pango lineage: AY.1/AY.2/AY.3; also known as "Delta Plus"). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

DETAILS

  • Tag: C-10×His
  • Host: HEK293
  • Size: 50ug
  • Buffer: PBS
  • Format: Powder
  • Purity: 95%
  • Storage: -20℃
  • Category: Viral Proteins
  • Molecule: Spike protein
  • Accession: QHD43416.1
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Exp Region: Val 16 - Pro 1213
  • Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.8 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 137.8 kDa
  • Shipping Condition: RT
  • Species Reactivity: SARS-CoV-2
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html