Anti-PCNA Monoclonal Antibody (Clone: PC 10)

DESCRIPTION

Proliferating cell nuclear antigen(PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.

DETAILS

Gene: Pcna
App Id: WB,IHC-P,IHC-F,ICC
Antigen: Proliferating cell nuclear antigen
Gene Id: 25737
Isotype: Mouse IgG2a
Clone Name: PC 10
Uniprot Id: P04961
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Ascites
Reactivity Id: Human
Product Content: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Alternative Name: ProlifeRating cell nuclear antigen; PCNA; Cyclin; Pcna
Application Note: Western blot : 2µg/ml; Immunohistochemistry(Paraffin-embedded Section) : 0.4-1µg/ml; Immunohistochemistry(Frozen Section) : 0.4-1µg/ml; Immunocytochemistry : 1µg/ml;
Storage Condition: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Immunogen Information: Protein A fusion protein.