DESCRIPTION

• Mw: 38.4 kDa
• Tag: mGFP
• Accn: NM_003908
• Type: Human Tagged ORF Clone
• Refseq: NM_003908.2
• Status: 3 Weeks*
• Symbol: EIF2S2
• Vector: pLenti-C-mGFP-P2A-Puro
• Domains: eIF2B_5
• Category: cDNA Clones
• Locus Id: 8894
• Orf Size: 999 bp
• Synonyms: eIF-2-beta; EIF2; EIF2B; EIF2beta; PPP1R67
• Refseq Orf: 1002 bp
• Uniprot Id: P20042
• Refseq Size: 2592 bp
• Availability: 3 Weeks
• Cytogenetics: 20q11.22
• Gene Summary: Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) The ORF insert of this clone is exactly the same as(RC201563).
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Chloramphenicol (34 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Puromycin