3165
Scientist.com Supplier
Bcl-G Antibody
ProSci
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DETAILS
- Buffer: Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.
- Isotype: IgG
- Storage: Bcl-G antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Category: Antibody
- Homology: Predicted species reactivity based on immunogen sequence: Bovine: (92%)
- Shipping: Blue Ice
- Clonality: Polyclonal
- Conjugate: Unconjugated
- Immunogen: Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G.The immunogen is located within the first 50 amino acids of Bcl-G.
- Swissprot: Q9BZR8
- User Note: Optimal dilutions for each application to be determined by the researcher.
- Background: Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.
- Specificity: Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
- Host Species: Rabbit
- Ncbi Gene Id: 79370
- Purification: Bcl-G Antibody is Ion exchange chromatography purified.
- Concentration: 1 mg/mL
- Modifications: None
- Ncbi Organism: Homo sapiens
- Physical State: Liquid
- Additional Names: Bcl-G Antibody: BCLG, BCLG, Apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14
- Application Note: Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
- By Research Area: Apoptosis
- Protein Accession: NM_030766
- Protein Gi Number: 13540528
- Ncbi Official Name: BCL2-like 14 (apoptosis facilitator)
- Positive Control 1: Cat. No. 1215 - U937 Cell Lysate
- Positive Control 2: Cat. No. 1416 - Mouse Testis Tissue Lysate
- Species Reactivity: Human,Mouse,Rat
- Tested Applications: ELISA,WB,IHC-P,IF
- Ncbi Official Symbol: BCL2L14