10345-H08H
Human Granzyme B / GZMB Protein (His Tag)
Sino Biological
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DESCRIPTION
A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.
DETAILS
- Tag: C-His
- Purity: ≥ 97 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.
- Source: HEK293 Cells
- Species: Human
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Measured by its ability to cleave a peptide substrate, tButyloxycaronylAlaAlaAspThioBenzylester (BocAADSBzl),in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB). (The enzyme needs to be activated by Recombinant Mouse Active Cathepsin C).
- Molecule: GZMB
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4
- Molecular Mass: The recombinant human Granzyme B consists of 240 amino acids and has a calculated molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhGranzyme B is approximately 35-40 kDa in SDS-PAGE under reducing conditions.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_004122.1
- Predicted N Terminal: Gly 19