DESCRIPTION

• Mw: 70.3 kDa
• Tag: mGFP
• Accn: NM_002670
• Type: Human Tagged ORF Clone
• Refseq: NM_002670.1
• Status: 3 Weeks*
• Symbol: PLS1
• Vector: pLenti-C-mGFP-P2A-Puro
• Domains: CH, EFh
• Category: cDNA Clones
• Locus Id: 5357
• Orf Size: 1887 bp
• Synonyms: DFNA76
• Refseq Orf: 1890 bp
• Uniprot Id: Q14651
• Refseq Size: 3736 bp
• Availability: 3 Weeks
• Cytogenetics: 3q23
• Gene Summary: Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Feb 2010]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) The ORF insert of this clone is exactly the same as(RC206291).
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Chloramphenicol (34 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Puromycin