32-1669
Periostin Recombinant Protein
Abeomics
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DESCRIPTION
Source : Escherichia Coli.
The OSF2 His-Tagged Fusion Protein Human is produced in E. coli, & its molecular weight is 75 kDa protein containing 648 amino acid residues of the human OSF-2 & 23 additional amino acid residues - HisTag, Xa - cleavage site.
Periostin is a disulfide linked 90 kDa, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts & is thought to be involved in osteoblast recruitment, attachment & spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) & bone morphogenetic protein (BMP-2). OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain & a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclinPeriostin mRNA is expressed in the developing mouse embryonic & fetal heart, & that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.
DETAILS
- Amino Acid: MGHHHHHHHH HHSSGHIEGR HMRNNHYDKI LAHSRIRGRD QGPNVCALQQ ILGTKKKYFS TCKNWYKKSI CGQKTTVLYE CCPGYMRMEG MKGCPAVLPI DHVYGTLGIV GATTTQRYSD ASKLREEIEG KGSFTYFAPS NEAWDNLDSD IRRGLESNVN VELLNALHSH MINKRMLTKD LKNGMIIPSM YNNLGLFINH YPNGVVTVNC ARIIHGNQIA TNGVVHVIDR VLTQIGTSIQ DFIEAEDDLS SFRAAAITSD ILEALGRDGH FTLFAPTNEA FEKLPRGVLE RFMGDKVASEALMKYHILNT LQCSESIMGG AVFETLEGNT IEIGCDGDSI TVNGIKMVNK KDIVTNNGVI HLIDQVLIPD SAKQVIELAG KQQTTFTDLV AQLGLASALR PDGEYTLLAP VNNAFSDDTL SMVQRLLKLI LQNHILKVKV GLNELYNGQI LETIGGKQLR VFVYRTAVCI ENSCMEKGSK QGRNGAIHIF REIIKPAEKS LHEKLKQDKR FSTFLSLLEA ADLKELLTQP GDWTLFVPTN DAFKGMTSEE KEILIRDKNA LQNIILYHLT PGVFIGKGFE PGVTNILKTT QGSKIFLKEV NDTLLVNELK SKESDIMTTN GVIHVVDKLL YPADTPVGND QLLEILNKLI KYIQIKFVRG STFKEIPVTV Y.
- Purification: Greater than 90% as determined by SDS-PAGE.
- Product Content: Filtered (0.4µm) & lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.
- Alternative Name: OSF-2||Periostin||Osteoblast Specific Factor 2||PN OSF-2||PDLPOSTN||POSTN||MGC119510||MGC119511||PN||RP11-412K4.1.
- Application Note: It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml & let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
- Storage Condition: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.