DK3-H5220-20ug DK3-H5220-200ug
Human Dkk-3 (R335G) Protein, His Tag
ACROBiosystems
DESCRIPTION
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.
DETAILS
- Tag: C-6×His
- Host: HEK293
- Size: 20ug
- Buffer: PBS, pH7.4
- Format: Powder
- Purity: 95%
- Source: Human Dkk-3 (R335G), His Tag (DK3-H5220) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Ile 350 (Accession # Q9UBP4-1 (R335G)).
- Storage: -20℃
- Category: Bio-Markers & CD Antigens
- Molecule: Dkk-3
- Accession: NP_001018067.1 (R335G)
- Conjugate: Unconjugated
- Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Exp Region: Ala 22 - Ile 350
- Swiss-prot: Q9UBP4-1 (R335G)
- Package Size: 20ug*1
- Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.0 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Endotoxin Level: 1.0 EU per μg
- Molecular Weight: 37.0 kDa
- Shipping Condition: RT
- Species Reactivity: Human
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