mouse monoclonal IgG1; p54/nrb Antibody (F-5) is an IgG1 κ mouse monoclonal p54/nrb antibody (also designated NONO antibody, Non-POU Domain Containing Octamer Binding antibody, NMT55 antibody, NRB54 antibody, Non-POU Domain-Containing Octamer-Binding Protein antibody, Protein Phosphatase 1 Regulatory Subunit 114 antibody, DNA-Binding P52/P100 Complex 52 KDa Subunit antibody, 54 KDa Nuclear RNA- And DNA-Binding Protein antibody, P54 antibody, or Non-POU Domain-Containing Octamer (ATGCAAAT) Binding Protein antibody) that detects the p54/nrb protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. p54/nrb Antibody (F-5) is available as both the non-conjugated anti-p54/nrb antibody form, as well as multiple conjugated forms of anti-p54/nrb antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.