Influenza A H3N2 (A/Darwin/9/2021) Hemagglutinin / HA trimer Protein (His Tag)(HPLC-verified)

DETAILS

Tag: C-His
Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90% as determined by SEC-MALS.
Source: HEK293 Cells
Species: H3N2
Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity: 1.Immobilized Influenza A H3N2 (A/Darwin/9/2021) Hemagglutinin / HA trimer Protein (His Tag)(HPLC-verified) (Cat:40859-V08H1) at 1μg/mL (100μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 2.5ng/mL-7ng/mL. 2.Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.6 μg/mL for 1% GRBC.
Molecule: HA
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Lead Time: 1-3 days
Formulation: Lyophilized from sterile PBS, pH 7.4.
Molecular Mass: The recombinant Influenza A virus ((A/Darwin/9/2021) (H3N2))Hemagglutinin / HA comprises 558 amino acids and has a predicted molecular mass of 62.34 kDa. It migrates as an approximately 87.6 kDa band in SDS-PAGE under reducing conditions.
Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Predicted N Terminal: Gln 17