Spike S1 (13-665), Fc Fusion, Avi-tag (SARS-CoV-2) Recombinant

DESCRIPTION

Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), GenBank Accession No. QHD43416.1, a.a. 13-665, fused at the C-terminus of the Fc portion of human IgG1, with a C-terminal Avi-tag™, expressed in a HEK293 expression system, MW=102 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

DETAILS

Aa: 13-665
Mw: 102 kDa
Tags: C-terminal Avi-tag
Genbank: QHD43416.1
Species: SARS-CoV-2
Uniprot: P0DTC2 SPIKE_WCPV
Shiptemp: -80°C (dry ice)
Synonyms: SARS-CoV s1, coronavirus spike S1
Warnings: Avoid freeze/thaw cycles.
Category: Coronavirus/Protein
References: 1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10.2. Walls, A. C. et al., Cell. 2002 Mar; 180:1-12
Description: Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), GenBank Accession No. QHD43416.1, a.a. 13-665, fused at the C-terminus of the Fc portion of human IgG1, with a C-terminal Avi-tag™, expressed in a HEK293 expression system, MW=102 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Supplied As: Aqueous buffer solution.
Unspsc Code: 12352202
Unspsc Name: Proteins
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Product Type: Protein
Biosafety Level: Not applicable (BSL-1)
Assay Conditions: SARS-CoV-2 S1 and ACE2 binding assay was done using BPS ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit (BPS Bioscience #79936). SARS-CoV-2 S1 protein and anti-human Fc-HRP were used instead of SARS-CoV-2 Spike protein (RBD) and anti-mouse Fc-HRP respectively. Non-specific His-tag protein was coated and used for a negative control.
Related Products: 11003, 100696, 100665, 100679
Storage Stability: At least 6 months at -80°C.
Scientific Category: Coronavirus