DESCRIPTION

• Aa: 2-186(end)
• Mw: 23 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution 
• Purity: ≥90%
• Genbank: NM_033360
• Species: Human
• Uniprot: P01116
• Shiptemp: -80°C (dry ice)
• Synonyms: GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed, KRAS2, RASK2
• Warnings: Avoid freeze/thaw cycles.
• Category: DNA Replication and Repair
• Constructs: KRAS (G12D) (His-TEV-2-186(end))-(GppNHp)
• Description: Recombinant human KRAS (KRAS proto-oncogene GTPase), isoform a, encompassing amino acids 2-186(end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays.
• Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT 
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: E. coli
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Assay was performed according to KRAS(G12C) Coupled Nucleotide Exchange Assay Kit (BPS Bioscience #78004) without SOS1/GTP mixture. KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant (BPS Bioscience #101312) and GppNHP loaded KRAS (G12D) (BPS Bioscience #101481) were titrated at various concentrations.
• Related Products: 100641, 100623, 100887, 78355
• Storage Stability: At least 6 months at -80°C. 
• Scientific Category: Ras Signaling