Total Bile Acid (TBA) Colorimetric Assay Kit

DETAILS

Storage: This product can be stored at 2~8°C for 12 months with shading light.
Inter Cv: 3.7
Intra Cv: 3.4
Shipping: Ice bag
Assay Time: 25 min
Result Type: Quantitative
Sensitivity: 2.05 μmol/L
Category Id Ii: Cell Metabolism Assays
Detection Type: Colorimetric method
Research Areas: Liver and Renal Function
Target Synonym: TBA
Test Principle: With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
Category Id Iii: Liver and Renal Function
Detection Range: 2.05-120 μmol/L
Tested Sample Types: serum, animal tissue
Detection Instrument: Microplate reader