Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

DESCRIPTION

Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

DETAILS

Tag: C-10×His & C-Avi | Native
Host: HEK293
Size: 25ug
Buffer: 50 mM Tris, 150 mM NaCl, pH7.5
Format: Powder
Purity: 90%
Source: Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His,Avitag&Tag Free (IT5-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # P06756-1 (ITGAV) & P18084-1 (ITGB5)).
Storage: -20℃
Category: MABSol® Biotin Labeled Proteins
Molecule: Integrin alpha V beta 5
Accession: NP_002201.1 (ITGAV) & NP_002204.2 (ITGB5)
Conjugate: Biotin-labeled
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)
Swiss-prot: P06756-1 (ITGAV) & P18084-1 (ITGB5)
Package Size: 25ug*1
Characteristics: Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-170 kDa (ITGAV) and 85-100 kDa (ITGB5) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 114.7 kDa (ITGAV) & 81.9 kDa (ITGB5)
Shipping Condition: RT
Species Reactivity: Human
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