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40200

EPHB2, His-tag Recombinant

BPS Bioscience

DESCRIPTION

Human EPHB2 (GenBank Accession No. NM_004442), a.a. 569-end with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

DETAILS

  • Aa: 569-end
  • Mw: 52 kDa
  • Tags: N-terminal His-tag
  • Format: Aqueous buffer solution
  • Genbank: NM_004442
  • Species: Human
  • Uniprot: P29323
  • Shiptemp: -80°C (dry ice)
  • Synonyms: EphB2, Ephrin Type-B Receptor 2, EPH-Like Kinase 5, ELK5, HEK5, Tyro5
  • Warnings: Avoid freeze/thaw cycles.
  • Category: Kinase/Receptor Tyrosine
  • References: 1. Macrae,M., et al., Cancer Cell 8 (2), 111-118 (2005). 2. Xu,H., et al., Am. J. Physiol. Renal Physiol. 288 (4), F855-F866 (2005).
  • Description: Human EPHB2 (GenBank Accession No. NM_004442), a.a. 569-end with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
  • Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.
  • Unspsc Code: 12352202
  • Unspsc Name: Proteins
  • Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
  • Host Species: insect
  • Product Type: Protein
  • Host Cell Line: Sf9 cells
  • Biosafety Level: Not applicable (BSL-1)
  • Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Invitrogen Zlyte Peptide 1) at room temperature for 1 hour.
  • Related Products: 40186, 40201, 78009, 78487
  • Specific Activity: 21.8 pmol/min/µg.
  • Storage Stability: >12 months at -80°C
  • Scientific Category: Kinase/Receptor Tyrosine