Polyclonal Antibody to Glial Fibrillary Acidic Protein, GFAP
Abeomics
Image
Figure-1: Immunofluorescent analysis of a section of mouse hippocampus stained with chicken pAb to GFAP,(34-1046), dilution 1:5,000 in green and costained with rabbit pAb to FOX3/NeuN,(34-1036), dilution 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.
Image
Figure-2: Western blot analysis of whole brain lysates using chicken pAb to GFAP,(34-1046), dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.
DETAILS
Gene:
GFAP
App Id:
WB, IF/ICC, IHC
Antigen:
Glial Fibrillary Acidic Protein, GFAP
Gene Id:
2670
Isotype:
Chicken, IgY
Format Id:
Conc. IgY prep.
Uniprot Id:
P14136
Host Animal:
Chicken
Clonality Id:
Polyclonal
Reactivity Id:
Human, Rat, Mouse, Cow, Pig, Horse
Product Content:
Antibody is supplied as an aliquot of concentrated IgY preparation
Application Note:
WB: 1:5,000. IF/ICC and IHC: 1:1,000-1:5,000
Storage Condition:
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information:
Native GFAP, purified from bovine spinal cord