Anti-CREB (Ser-271), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: CREB1
Host: Rabbit
Rrid: AB_10837645
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: CREB (Ser-271)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Cyclic AMP, CREB
Clonality: Rabbit Polyclonal
Immunogen: Phospho-CREB (Ser-271) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-271 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB. The site has some homology to the conserved site in ATF1 (Ser-198) and CREM (Ser-287).
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 43
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:200
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in the nervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calcium influx and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271 potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation for CREB-dependent transcription in response to genotoxic stress.
Uniprot Number Immunogen Species: P16220
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-271