DESCRIPTION

• Aa: full length
• Mw: 78 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_006258
• Species: Human
• Uniprot: Q13976
• Shiptemp: -80°C (dry ice)
• Synonyms: CGK1B, PRKG1B, cGMP-dependent, type I, cGKIb
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• References: 1. Bivalacqua T.J. et al., BJU Int. 2007 Jun,99(6):1488-94. 2. Lee J.H. et al., Retrovirology. 2007 Dec 13,4:91.
• Description: Human PKG1β (beta) (GenBank Accession No. NM_006258), full length with N-terminal His-tag, MW = 78 kDa, expressed in Baculovirus infected Sf9 cell expression system.
• Formulation: 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
• Specific Activity: 0.85 pmoles/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine