51031
SMYD2 (KMT3C) Chemiluminescent Assay Kit
BPS Bioscience
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DESCRIPTION
The SMYD2 Chemiluminescent Assay Kit is designed to measure SMYD2
activity for screening and profiling applications. The key to the SMYD2 Chemiluminescent Assay Kit is a highly specific antibody that
recognizes a methylated residue of Histone H3. With this kit, only three simple steps are required
for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample
containing assay buffer and the SMYD2 methyltransferase enzyme. Next, primary antibody is
added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition
of the HRP substrate to produce chemiluminescence that can then be measured using a
chemiluminescence reader.
DETAILS
- Notes: MATERIALS REQUIRED BUT NOT SUPPLIED: TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20) Luminometer or fluorescent microplate reader capable of reading chemiluminescence Adjustable micropipettor and sterile tips Rotating or rocker platform
- Shiptemp: -80°C (dry ice)
- Synonyms: KMT3C
- Warnings: Avoid freeze/thaw cycles.
- Category: Methyltransferase/Assay Kit
- References: Brown MA, et al. Mol. Cancer 2006; 5:26.
- Description: The SMYD2 Chemiluminescent Assay Kit is designed to measure SMYD2 activity for screening and profiling applications. The key to the SMYD2 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and the SMYD2 methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
- Methodology: Chemiluminescent
- Supplied As: The SMYD2 Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 substrate, antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase
- Unspsc Code: 41105332
- Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
- Applications: Great for studying enzyme kinetics and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 51015, 51016, 51014, 27604-1, 27605-1
- Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
- Storage Stability: One year from date of receipt when stored as directed.
- Scientific Category: Methyltransferase
- Instructions for Use: See product datasheet for detailed assay protocol.