80501
PARP1, GST-tag Recombinant
BPS Bioscience
DESCRIPTION
Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal GST-tag, MW = 140 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
DETAILS
- Aa: full length
- Mw: 140 kDa
- Tags: N-terminal GST-tag
- Format: Aqueous buffer solution
- Purity: ≥80%
- Genbank: NM_001618
- Species: Human
- Uniprot: P09874
- Shiptemp: -80°C (dry ice)
- Synonyms: poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1
- Warnings: Avoid freeze/thaw cycles.
- Category: PARP/Enzymes
- References: 1. Aguilar-Quesada R, et al., 2007. Curr Med Chem. 14(11):1179-87.2. Pacher P. and Szabó C. 2007. Cardiovasc Drug Rev. 25(3):235-60. Application Reference(s): De Vos, M., et al. J Biol Chem. 2014 Jun 6;289(23):16223-38. doi: 10.1074/jbc.M113.527424. Poly(ADP-ribose) polymerase 1 (PARP1) associates with E3 ubiquitin-protein ligase UHRF1 and modulates UHRF1 biological functions (2014) Larsson, E.A., et al. J Med Chem. 2013 Jun 13;56(11):4497-508. doi: 10.1021/jm400211f. Fragment-based ligand design of novel potent inhibitors of tankyrases (2013)
- Description: Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal GST-tag, MW = 140 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT.
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Product Type: Protein
- Biosafety Level: Not applicable (BSL-1)
- Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
- Assay Conditions: Enzyme reaction is conducted using BPS's PARP1 Assay Kit (Catalog #80551) at room temperature for 1 hour.
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: PARP