DESCRIPTION

• Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com
• Uniprot: Q9NQU5
• Shiptemp: -80°C (dry ice)
• Synonyms: Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6 (PAK-6), PAK5
• Warnings: Avoid freeze/thaw cycles
• Category: Kinase/Assay Kit
• Background: PAK6 (p-12-activated kinase 46 is a member of the PAK family of serine/threonine kinases. The PAK family is highly evolutionary conserved and regulates cell survival, apoptosis, cytoskeleton dynamics, and other critical cellular functions. PAK6 belongs to group II, which can be activated by Cdc42 (cell division control protein 42) only as opposed to group I, which depend on either Rac1 or Cdc42.I It is ubiquitously expressed, participating in cytoskeleton remodeling, cell motility, apoptosis, and metastasis. It has been found at high levels in prostate and colon cancer and hepatocellular carcinoma. It has been correlated with resistance to cancer therapy with 5-flurouracil, docetaxel and radiation. Recently it has been shown that PAK6-related resistance to oxaliplatin treatment is due to an increase in HR (homologous recombination) DNA repair, by translocating from the cytoplasm to the nucleus and activating ATR (ataxia telangiectasia and Rad-3 related protein) and CHK1 (checkpoint kinase 1). The development of specific inhibitors targeting PAK4, to be used alone or as combination therapy, will greatly benefit the cancer therapy field.
• References: Huang W., et al., 2022 Cell Death & Disease. 13: 658.
• Description: The Chemi-Verse™ PAK6 Kinase Assay Kit is designed to measure PAK6 (p21-activated kinase 6) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant PAK6 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
• Methodology: Luminescent
• Unspsc Code: 41105332
• Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
• Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 82181, 40148, 40004, 82094
• Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
• Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
• Scientific Category: Kinase