DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: Suppressor Of Variegation 4-20 Homolog 1 , KMT5B, SUV420H1
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Assay Kit
• References: Dillon S.C., et al. 2005. Genome Biology 6:227.
• Description: The SUV4-20H1 Chemiluminescent Assay Kit is designed to measure SUV4-20H1 activity for screening and profiling applications. The key to the SUV4-20H1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes a methylated residue of Histone H4. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
• Methodology: Chemiluminescent
• Supplied As: Kit comes in a convenient format, with 96-well plate precoated with histone H4 substrate, reagents, and purified SUV4-20H1 enzyme for 96 enzyme reactions.
• Unspsc Code: 41105332
• Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Methyltransferase
• Instructions for Use: See assay kit datasheet for detailed protocol.