DESCRIPTION

• Tag: Tag Free
• Accn: NM_001286178
• Type: Mouse Untagged Clone
• Refseq: NM_001286178.1, NP_001273107.1
• Status: 2 Weeks*
• Symbol: Eif4e1b
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 218268
• Synonyms: Eif4eloo; Gm273
• Refseq Orf: 750 bp
• Uniprot Id: Q3UTA9
• Insert Size: 750 bp
• Refseq Size: 1848 bp
• Availability: 2 Weeks
• Cytogenetics: 13 B1
• Gene Summary: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice site in the 5'-terminal exon, and initiates translation from an alternate start codon, compared to variant 1. The encoded isoform (3) has a longer N-terminus, compared to isoform 1.
• Sequence Data: Fully Sequenced ORF ( show hide ) >MC226573 representing NM_001286178 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCCACTTCTGAGATGAACAAAGTTGAGGGTGGAGGGCATAAGGAGGAGGTGGTGGTGAAAGAAAAGG AGGTAGTGAAAGAAAAGCCATCAGAAGCAACTGCGGAGGGAGTCCAAGCAGGAGAAGCCAAAGACCTTCC TGGCTCCCTTAAGACTCAGAGGAGGAAGGCCCACAGAGAACATCCACCAGAGGTCCTGAGCAAGCTACAC CCCTTGCAGTACAGGTGGGTTCTGTGGTTCTTCAAGAATGACCGAAGCCGGGCCTGGCAGGACAACCTGC AGCTAGTCACCAAGTTTAACACTGTGGAGGACTTCTGGGCGGTGTACAGCCATATCAAGCTGGCCAGCAA GCTCTCTTCCGGCTGTGACTATGCCCTGTTCAAGGAAGGCATCCTGCCCATGTGGGAAGACAACAGAAAC AAGCAGGGTGGCCGCTGGCTGCTCAGCATTGACAAACAACTGCGCCACTTCGAACTGGACCGTCTGTGGC TGGAGACGTTGCTGTGTCTGGTTGGGAATTGTTTTGAGGAATACAGCAGGGAAGTGTGCGGTGCTGTCGT GAACATCCGCACGAAGAGGGACAAGATTGCCCTGTGGACGAGTGAAGCAGAGGACAAAGCCGGAGTGATG CAGATCGGGCAAATATACAAAGAGCGCCTGGGCATCTCTACGAAGACCATCATTGGGTACCAGGCCCATG CAGACACTGCTGCCAAGAGCAACAACTTAGCAAACAAGTTTGTGGTGTGA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin