PLK3, His-Tag Recombinant

DESCRIPTION

Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal His-tag, MW=36.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells Purity:≥80% by SDS-PAGE. Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP.

DETAILS

Aa: 58-339
Mw: 36.7 kDa
Tags: N-terminal His-tag
Format: Aqueous buffer solution
Genbank: NM_004073
Species: Human
Uniprot: Q9H4B4
Shiptemp: -80°C (dry ice)
Synonyms: CNK, FNK, PRK, polo-like kinase 3
Warnings: Avoid freeze/thaw cycles.
Category: Kinase-Serine/Threonine/Protein
Background: PLK3 or Polo-like kinase 3 is a cytokine-inducible kinase that is a member of the Polo family of serine/threonine protein kinases. PLK3 contains both a catalytic domain and a putative regulatory domain and plays an important role in the regulation of cell cycle progression and tumorigenesis. PLK3 is closely associated with centrosomes in several human cell lines and its localization is dependent on the integrity of microtubules. Hence, PLK3 is also involved in regulating microtubule dynamics and centrosomal function.
References: 1. Li, Z., et al., J. Biol. Chem. 280 (17), 16843-16850 (2005). 2.Xie,S., et al., Oncogene 23 (21), 3822-3829 (2004).
Description: Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal His-tag, MW=36.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells Purity:≥80% by SDS-PAGE. Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP.
Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, 50% glycerol and 1 mM DTT.
Unspsc Code: 12352202
Unspsc Name: Proteins
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Host Species: insect
Product Type: Protein
Host Cell Line: Sf9 cells
Biosafety Level: Not applicable (BSL-1)
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of substrate per minute at pH 7.5 and 30°C.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200µM ATP, 0.01% Brij-35 and 2 µM substrate (SER/THR 16, Invitrogen) at room temperature for 1 hour.
Related Products: 50302, 40034, 40033, 40236, 78487
Specific Activity: 350 pmol/min/µg
Storage Stability: At least 6 months at -80°C.
Scientific Category: Kinase-Serine/Threonine