DESCRIPTION

• Aa: 50-140
• Mw: 36 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_002880
• Species: Human
• Uniprot: P04049
• Shiptemp: -80°C (dry ice)
• Synonyms: C-Raf, NS5
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/protein
• References: 1. Rooij, J., et al., Oncogene. 1997;14:623-625.2. Bhaswati, P., et al., Nature Genetics. 2007;39:1007-1012.
• Description: Human Ras Binding Domain (RBD) of Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1) also known as C-Raf and NS5 with N-terminal GST-tag, GenBank Accession No. NM_002880, a.a. 50-140, expressed in an E. coli expression system. MW=36 kDa.
• Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.
• Supplied As: Aqueous buffer solution.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for studying RAS/RAF binding study as well as inhibitor screening for RAS/RAF interaction.
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: GppNHp (or GDP) loaded His-tag KRAS(G12C) at various concentrations (BPS Bioscience #100641 and BPS Bioscience #100640) was incubated with 2.5 nM GST-tag RBD-RAF1 for 1 hour at room temperature. KRAS(G12C)/RBD-RAF1 binding was analyzed by AlphaScreening using GSH-Acceptor beads and Nickle Donor beads (PerkinElmer).
• Related Products: 40008, 40065, 48688, 79570, 78487
• Storage Stability: At least 6 months at -70°C.
• Scientific Category: Kinase