DESCRIPTION

• Aa: 28-232(end)
• Mw: 28 kDa
• Tags: none
• Format: Lyophilized solid
• Genbank: NM_005450
• Species: Human
• Uniprot: Q13253
• Shiptemp: -80°C (dry ice)
• Synonyms: Noggin, symphalangism 1, SYM1, synostoses (multiple) syndrome 1, SYNS1
• Warnings: Avoid freeze/thaw cycles.
• Category: Cytokine
• Background: Noggin is one of a group of proteins that act as secreted antagonists of BMP activity. BMPs are essential for osteogenesis and organogenesis during embryonic development, and also play a role in tissue healing in adults. Noggin inhibits BMP activity by binding to them with high affinity, blocking their ability to bind to their receptor.
• References: 1. Tao, Y.X., et al. (2010). Indian J. Exp. Biol. 48(5):444-52.2. Mfopou, J.K., et al. (2010). Gastroenterology. 138(7):2233- 2245. 3. Chaturvedi, G., et al. (2009). Cell Prolif. 42(4):425-33
• Description: Human Noggin, also known as symphalangism 1 (SYM1) or synostoses (multiple) syndrome 1 (SYNS1), GenBank Accession No. NM_005450, a.a. 28-232(end), disulfide-linked homodimer. MW=~28kDa (monomer), expressed in Freestyle 293F cells.
• Formulation: Lyophilized from a 0.2 µm filtered 0.1 mg/ml solution of 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
• Product Type: Protein
• Reconstitution: Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0, 1.2 M NaCl, 2.7 mM KCl, 0.1% BSA. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
• Biosafety Level: Not applicable (BSL-1)
• Endotoxin Level: < 1EU per µg of Noggin as determined by the LAL method.
• Storage Stability: ≥12 months (lyophilized) at -20°C. Store reconstituted protein in aliquots at -20°C to -70°C for up to 6 months. Avoid freeze/thaw cycles.
• Biological Activity: Measured by Noggin's ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is <20 ng/ml in the presence of 30 ng/ml of human BMP-4.
• Scientific Category: Cytokine/Growth Factors