Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (Monomer, MALS verified)

DESCRIPTION

Ovalbumin (OVA) can induce both humoral and cellular immune responses based on well-characterized peptide epitopes. The OVA257-264 octapeptide was one of the first OVA epitopes to be characterized, it has an amino acid sequence SIINFEKL, which is recognized by cytotoxic T lymphocytes. ovalbumin (Ova) was presented to T cells if it was released from pinosomes into the cytosol by osmotic lysis of pinosomes. Immunization with the adjuvanted SIINFEKL peptide induces long-lasting CD8+ T cell immunity in mice.

DETAILS

Tag: C-10×His & C-Avi
Host: HEK293
Size: 25ug
Buffer: PBS, pH7.4
Format: Powder
Purity: 95%
Source: Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein (H2A-M82E6) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide (Accession # P01901 (H-2Kb) & P01887 (B2M) & SIINFEKL).
Storage: -20℃
Category: MABSol® Biotin Labeled Proteins
Molecule: H-2Kb & B2M & OVA (SIINFEKL)
Conjugate: Biotin-labeled
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Gly 22 - Thr 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide
Swiss-prot: P01901 | P01887 | SIINFEKL
Package Size: 25ug*1
Characteristics: Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 39.7 kDa and 15.7 kDa. The protein migrates as 48-53 kDa and 17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 39.7 kDa and 15.7 kDa
Shipping Condition: RT
Species Reactivity: Mouse
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