DESCRIPTION

• Aa: 627-1084 (end)
• Mw: 76 kDa
• Tags: N-terminal GST-tag, C-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_006037
• Species: Human
• Uniprot: P56524
• Shiptemp: -80°C (dry ice)
• Synonyms: histone deacetylase 4, HDAC-4
• Warnings: Avoid freeze/thaw cycles.
• Category: Deacetylase/Protein
• References: 1. Leong, H. et al. (2005) Mol. Endocrinol. 19, 2930-2942. 2. Zhao et al., (2005) Mol. Cell. Biol. 25, 8456-8464. 3. Wang, C., et al. J Med Chem. 2014 Oct 9;57(19):7999-8009. doi: 10.1021/jm500872p. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
• Description: Human HDAC4 (GenBank Accession No. NM_006037), a.a. 627-1084 (end) with N-terminal GST-tag and C-terminal His-tag, MW= 76 kDa, expressed in baculovirus expression system.
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC4. Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
• Specific Activity: ≥100,000 pmol/min/µg.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Deacetylase