Anti-Axl/UFO (Extracellular region) Antibody

DETAILS

Form: Protein G Purified
Gene: AXL
Host: Mouse
Rrid: AB_3068355
Type: Primary Antibody
Clone: M047
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Axl/UFO (Extracellular region) Antibody
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Tyrosine-protein kinase receptor, UFO, Axl, Tyro7, ARK,
Clonality: Mouse Monoclonal
Immunogen: Clone (M047) was generated from a recombinant protein that included the extracellular region of human Axl protein.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: Clone M047 mouse monoclonal antibody detects a 140 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human A549, NCI-H1915, and MDA-MB-231 carcinomas. The antibody detects Axl in membranes and cytoplasm in MDA-MB-231 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Applications: WB, ICC, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 140
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human
Target Description: The Axl/UFO receptor tyrosine kinase (RTKs) family includes Axl/UFO/Tyro7, Sky/Tyro3, and c-Mer/Tyro12. These RTKs have a conserved intracellular tyrosine kinase domain and extracellular domains that include immunoglobulin-like and fibronectin-type moieties similar to those found in cell adhesion molecules. The ligand for these receptors is the vitamin Kdependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor. Upon binding to its receptor, Gas6 activates phosphatidylinositol 3- kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB. Axl is overexpressed in several cancers, including breast, lung, liver, colon, gastric, ovarian, pancreatic, and glioblastoma. The Axl/Gas6 signalling pathway has been shown to drive cancer cell survival, proliferation, migration and invasion, and several therapeutic strategies are being developed to regulate Axl cell signaling.
Uniprot Number Immunogen Species: P30530
Post Translational Modification Type: GOmAb