DESCRIPTION

• Aa: CDK5 1-292 (end), p25 99-307 (end)
• Mw: 60 kDa (CDK5), 50 kDa (p25)
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_004935, NM_003885
• Species: Human
• Uniprot: Q00535 (CDK5), Q15078 (p35)
• Shiptemp: -80°C (dry ice)
• Synonyms: Cyclin-Dependent Kinase-5, CDK5: PSSALRE p25: CDK5R1; CDK5P35, CDK5R, NCK5A, p23, p35, p35nck5a
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• Background: CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
• References: 1. Tang, D. et al: Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16.2. Dhavan, R. et al: A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59.
• Description: Complex of recombinant full length human CDK5 (GenBank Accession No. NM_004935), a.a. 1-292 (end) with N-terminal GST-tag and p25 (GenBank Accession No. NM_003885), a.a. 99-307 (end) with N-terminal GST-tag. Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK5 MW=60 kDa, p25 MW=50 kDa.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 0.04% Tween-20, and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Assay was done in a Kinase buffer using Histone H1 (BPS cat. #52043) as a substrate with 20 µM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).
• Related Products: 40095, 79600, 78487
• Specific Activity: 312 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine