40105
CDK5/p25, GST-tag Recombinant
BPS Bioscience
DESCRIPTION
Complex of recombinant full length human CDK5 (GenBank Accession No. NM_004935), a.a. 1-292 (end) with N-terminal GST-tag and p25 (GenBank Accession No. NM_003885), a.a. 99-307 (end) with N-terminal GST-tag. Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK5 MW=60 kDa, p25 MW=50 kDa.
DETAILS
- Aa: CDK5 1-292 (end), p25 99-307 (end)
- Mw: 60 kDa (CDK5), 50 kDa (p25)
- Tags: N-terminal GST-tag
- Format: Aqueous buffer solution
- Genbank: NM_004935, NM_003885
- Species: Human
- Uniprot: Q00535 (CDK5), Q15078 (p35)
- Shiptemp: -80°C (dry ice)
- Synonyms: Cyclin-Dependent Kinase-5, CDK5: PSSALRE p25: CDK5R1; CDK5P35, CDK5R, NCK5A, p23, p35, p35nck5a
- Warnings: Avoid freeze/thaw cycles.
- Category: Kinase-Serine/Threonine/Protein
- Background: CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
- References: 1. Tang, D. et al: Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16.2. Dhavan, R. et al: A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59.
- Description: Complex of recombinant full length human CDK5 (GenBank Accession No. NM_004935), a.a. 1-292 (end) with N-terminal GST-tag and p25 (GenBank Accession No. NM_003885), a.a. 99-307 (end) with N-terminal GST-tag. Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK5 MW=60 kDa, p25 MW=50 kDa.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 0.04% Tween-20, and 20% glycerol
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: Assay was done in a Kinase buffer using Histone H1 (BPS cat. #52043) as a substrate with 20 µM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).
- Related Products: 40095, 79600, 78487
- Specific Activity: 312 pmol/min/µg
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Kinase-Serine/Threonine