DESCRIPTION

• Form: Lyophilized powder
• Host: Rabbit
• Clone: NA
• Label: ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
• Purity: Affinity purified on immobilized antigen.
• Target: Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: 25360
• Isotype: Rabbit IgG
• Homology: Mouse, human - identical
• Is Toxin: No
• Sequence: ETLQAFDSHYDYTVC, corresponding to amino acid residues 376-390 of rat TSHR
• Synonyms: Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
• Accession: P21463
• Clonality: Polyclonal
• Lead Time: 1-2 Business Days
• Reactivity: Human|Rat|Mouse
• Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
• Applications: IF|IHC
• Preservative: 1% BSA, 0.05% NaN3
• Reconstitution: 50 µl double distilled water (DDW).
• Blocking Peptide: BLP-TR006
• Negative Control: RIC-001-AG
• Positive Control: NA
• Immunogen Location: Extracellular, N-terminus
• Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
• Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
• Immunogen Source Species: Rat
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
• Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
• Applications May Also Work In: IF|IHC
• Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
• Product Page - Scientific Background: Thyroid stimulating hormone (TSH), a glycoprotein hormone composed of α and β chains, is produced by thyrotrope cells of the anterior pituitary1. The TSH receptor (TSHR) plays a prominent role in thyroid physiology and disease. The established biological function of TSHR in the thyroid gland is to regulate synthesis and secretion of thyroid hormones from follicular thyroid cells; it also plays an important role in controlling the growth and development of the thyroid gland2.TSHR is a G-protein coupled receptor and shares the classic structure of the serpentine receptor family (seven membrane spanning segments, an extracellular N-terminus and an intracellular carboxy terminal)3.TSHR is primarily expressed in the epithelial cells of the thyroid follicles, but TSHR mRNA and protein have been detected in a variety of cell types including brain, testes, kidney, heart, bone, thymus, lymphocytes, adipose tissue and fibroblasts4.Chronic stimulation of the TSHR leads to over activation of the cAMP pathway that in turn causes thyroid hyperplasia and hyperthyroidism. This process occurs in Graves' disease (GD) in which thyroid stimulating antibodies (TSAB) bind the receptor and mimic the action of TSH5.
• Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #ATR-006), and immunohistochemical staining (labeled antibody).
• Application Dilutions Western Blot Wb: Contact Alomone for information
• Antibody Concentration After Reconstitution: 1 mg/ml
• Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:60